Transplantation of artificial esophagus using bFGF, keratinocyte, polyglicolic acid felt and polyglactin mesh stent

使用人造食道进行食道置换被认为是困难的。人们已经尝试用各种人造食道来取代食道缺陷。在本研究中，我设计了一种新型的人工食道，由碱性成纤维细胞生长因子释放片、角质形成细胞、聚甘油酸毡和聚乳酸网状支架组成。我们在体外成功地研制了这种新型人工食道，并替代了狗的颈、胸段食道。术后4周行静脉高营养治疗。结果，人工食道组织成功地替代了食道缺损。显微镜下，替换的人工食道完全上皮化。然而，替代食道狭窄的发生率很高。在胸段食道置换术中，由于手术压力和/或手术并发症，所有的狗在手术后一个月内死亡。我们没有使用新型人工食道进行移植，然而，这些问题的解决将为临床上的食道重建带来希望。

Esophageal replacement using an artificial esophagus is considered difficult. Attempts have been made to replace esophageal defects with a variety of artificial esophagus. In this research, I designed a new type of artificial esophagus, consisted of bFGF-releasing sheet, keratinocyte, polyglicolic acid felt, and polyglactin mesh stent. We successfully developed this new type of artificial esophagus in vitro, and replaced for cervical and thoracic esophagus in dogs. Intravenous hyperalimentation was performed for 4 weeks after the surgery. As results, artificial esophageal tissue successfully replaced the esophageal defect. Microscopically, complete epithelization was observed in the replaced artificial esophagus. However, a stenosis in replaced esophagus occurred frequently. In the thoracic esophageal replacement, all dogs died after surgery within a month due to surgical stress and/or surgical complications. We did not transplant using a new type of artificial esophagus, however, the solution these problems will hold promise for esophageal reconstruction in a clinical setting.