A novel diagnostic method using C-reactive protein genetic polymorphism in patients with thoracic esophageal cancer and its mechanisms

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We inoculated subcutaneously NR-S1M metastatic cells into the backs of C3H/HeN mice. Concurrently, 1 μg of recombinant mouse CRP or PBS were injected subcutaneously every 3 days for 5 weeks, after which the mice were killed for evaluation. We evaluated lymph node metastasis and lymphangiogenesis in the implanted tumor using immunohistochemical analysis with anti-lymphatic vessel endothelial hyaluronan receptor-1 antibodies. Immunohistochemical analysis confirmed inguinal lymph node metastasis in 70% of control mice, but in only 30% of mice in the CRP group. Moreover, tumoral lymphangiogenesis was decreased in the CRP group). CRP appears to inhibit tumoral lymphangiogenesis and lymph node metastasis in mice.