Correlation of motility and neuronal characteristics in intestinal allograft chronic rejection

[目的]探讨大鼠小肠移植术后慢性排斥反应中肠动力与神经元完整性的关系。[材料与方法]采用同种异体移植方法，受体分为3组：第1组：采用同种异体移植方法，从Brown Norway（BN）移植到刘易斯（LEW），FK506 2mg/kg，第0-5天肌肉注射，建立慢性排斥反应模型。第2组：也以2 mg/kg剂量肌肉注射FK 506进行同种异体SBTx，第0 - 9天，作为长期功能模型。第3组：BN-BN同基因移植，不加FK506。在第80天，在移植物上设置三个微型应变式力传感器以评估体内运动模式。取移植物全标本行亚铜兰染色和免疫组化染色，观察神经元细胞和Cajal间质细胞（ICC）的数量。对移植肠行HE组织学检查， ...更多信息 定义慢性排斥反应。[结果]移植肠组织学观察：第1组移植肠血管壁增厚，H-E染色见肌层增厚，符合慢性排斥反应的定义;移植肠的运动功能MMC Ⅲ期参数在各组间有特征性变化。MMC III期的持续时间和间期在第1组和第3组之间有显著性差异。（同系模型对慢性排斥模型持续时间：6.2 ± 1.8（min）对5.7 ± 1.7（min）（p <0.01），间隔：19.1 ± 5.3（min）对16.7 ± 4.3（min）（p <0.0001））标记神经元细胞和ICCWe仍在研究中。[结论]本研究提供了慢性排斥移植肠动力学的重要特征。运动参数包括移行性复合运动的持续时间和间隔时间，表明慢性排斥反应的诊断具有关键作用。我们仍在研究神经元状况的改变，但我们相信这些方法将揭示与移植物运动障碍的相关性。少

【Aim】The aim of this study was to elucidate correlation between motility and neuronal integrity for chronic rejection after small bowel transplantation in rats.【Material and Method】Orthotopic SBTx was performed and recipients were divided following three groups; group 1: Allogenic SBTx from Brown Norway (BN) to Lewis (LEW) was done with FK506 at a dose of 2mg/kg, intramuscularly, day0-5, which is considered as chronic rejection model. group 2: Allogenic SBTx was also done with FK506 at a dose of 2mg/kg i.m., day0-9, which served as long-term functional model. group 3: Syngenic transplant was prepared from BN to BN without FK506. On day 80, three miniature strain gauge force transducers were set on the graft to evaluate motility pattern in vivo. Wholemount of the grafts were applied into cuprolinic blue staining and immunohistochemistry to assess population of neuronal cell and interstitial cells of Cajal (ICC). H-E histological examination of the intestinal grafts was also performed to … More define the chronic rejection.【Results】Histology of the Intestinal GraftsIn group 1, arterial wall thickening as vascular sclerotic findings and thickening in the muscular layer was observed in H-E staining which was fit in the definition of chronic rejection. Whereas the other 2 groups had no finding of rejection.Motility of the Intestinal GraftsThe parameter of MMC phase III has a characteristic alteration among these groups. The duration and interval of MMCphase III has a significant difference between group 1 and group 3. (syngenic model vs chronic rejection modelDuration ; 6.2±1.8 (min) vs 5.7±1.7 (min) (p<0.01), Interval ; 19.1±5.3(min) vs 16.7±4.3(min) (p<0.0001))Labeling-neuronal cells and ICCWe are still under investigation.[Conclusion] This study provides important characteristics on motility in chronically rejected intestinal allogtafts. The motility parameter including duration and interval of migrating motor complex indicates a key role for diagnosing chronic rejection. We are still under investigation on the alteration of the neuronal condition, but we believe these approaches will reveal the correlation with dysmotility of the grafts. Less