Clock gene response against skelton type entrainment stimulation in mice

本实验的目的是阐明光和非光夹带的机制，如食物夹带在小鼠。首先，我们研究了时钟基因表达的昼夜节律模式在肝脏下一小时的光刺激在早上和一小时的光刺激在晚上。这种光照时间表可以诱导自发活动节律，也可以诱导肝脏dock基因表达节律。因此，骨架照明时间表可以像通常的12小时光暗时间表一样携带昼夜节律码头。接下来，我们研究了时钟基因表达的昼夜节律模式在肝脏下2小时喂养在早上和2小时喂养在晚上。这种饲喂方式可以诱导肝钟基因表达节律，其模式与自由饲喂组非常相似。因此，每天早晚两次喂食可以诱导外周昼夜节律。试验三改变早晚投喂量的比例。早晨投饵量越大，对dock基因表达的时相影响越小。但如果在晚上投喂量较大，则dock基因表达节律的时相明显延迟。综上所述，这些结果表明，在早上的食物摄入是重要的，以保持码头基因表达的外周器官的相位。

The aim of this experiment is to elucidate the mechanism of skeleton-type photic and non-photic entrainment like food entrainment in mice. First we examined the circadian pattern of clock gene expression in the liver under one-hr light stimulation in the morning and one-hr light stimulation in the evening. This lighting schedule could entrain the locomotor activity rhythm and also liver dock gene expression rhythm. Thus, skeleton lighting schedule can entrain the circadian dock just like usual 12-hr light-dark schedule. Next, we examined the circadian pattern of clock gene expression in the liver under 2-hr feeding in the morning and 2-hr feeding in the evening. This feeding schedule could entrain the liver clock gene expression rhythm, and its pattern was very similar to adlib feeding group. Thus, two feeding times per day with morning and evening could entrain the peripheral circadian rhythm. In the third experiment, we changed the ratio of feeding volume in the morning and evening. If the more feeding volume was applied in the morning, there was little effect on the phase of dock gene expression. However, if the more feeding volume was applied in the evening, phase of dock gene expression rhythm was strongly delayed. Taken together these results suggest that food intake in the morning is important to keep the phase of dock gene expression the peripheral organs.