Establishment of therapeutic strategy for degenerative muscular disease by embryonic stem mils

目前尚无治疗肌营养不良症的基本方法，因此这些患者的命运是严重的。为了改善这些患者的生活质量，各种治疗策略的发展正在调查中。胚胎干细胞是一种很有前途的候选细胞。在本研究中，我们发展了从小鼠胚胎干细胞中制备和分离骨骼肌干细胞卫星细胞的方法，并在数学凝胶上继续培养。在这个过程中，分化的骨骼肌的形成得到了证实。同时，通过特异性抗体SM/C-2.6鉴定了骨骼肌干细胞，即卫星细胞。经鉴定的细胞在体外可分化为肌肉细胞。同时，将这些细胞移植到MDX小鼠(肌营养不良症小鼠)体内，导致了肌肉纤维的形成。此外，机械性肌肉损伤导致SM/C-2.6阳性细胞扩增，导致骨骼肌干细胞数量增加。此外，这些细胞二次移植的成功也揭示了肌肉干细胞的明确特性。

There is no fundamental therapeutic method far muscular dystrophy, therefore the fates of these patients axe serious. To improve the quality of life for these patients, the development of various therapeutic strategiesis is under investigation. Embryonic stem cell is one promising candidate. In this research, we developed the method to produce and isolate skeletal muscle stem cells, satellite cells, from mouse ES cells Following the formation of embryoid bodyies, the culture was continued on math gel. During this process, the formation of differentiated skeletal muscles was confirmed. Along with this, skeletal muscle stem cells, satellite cells, were identified by specific antibody, SM/C-2.6. Identified cells differentiate into muscle, cell in vitro. Simultaneously, transplantation of these cell in MDX mice (muscular dystrophy mice) resulted into the formation of muscle fibers. Furthermore, mechanical muscle damage led to the amplification of SM/C-2.6 positive cells, resulting in the increased number of skeletal muscle stem cells. In addition, success of second transplantation of these cells revealed the definite character of muscle stem cells.