Nonlinear Raman microscopy to detect membrane protein

我们开发了一种多焦激励非线性拉曼显微镜，使用微透镜阵列扫描仪进行实时分子成像。用两个高度可控的ps锁模激光器（抖动30fs，逐点波长扫描300ms以内）对样品进行平行曝光，并用图像传感器进行平行检测，实现实时成像。我们演示了活细胞的实时拉曼成像（帧率为10fps）。我们还将所开发的系统与激光烧蚀相结合，对细胞膜进行破坏，并演示观察细胞膜的修复过程。我们在非线性拉曼显微镜系统中加入偏振模式控制系统，通过控制聚焦点上的电磁场方向来检测膜蛋白。用液晶成像证实了选择性检测有序分子的可能性。

We developed a multi-focus excitation nonlinear Raman microscope using a microlens array scanner for real-time molecular imaging. Parallel exposure of a specimen with light from two highly controlled ps mode-locked lasers (jitter of 30fs, point-by-point wavelength scan within 300ms) and parallel detection with an image sensor enabled real-time imaging. We demonstrated real-time Raman imaging of living cells (frame rate of 10fps). We also combined the developed system with the laser ablation to destruct the cell membrane, and demonstrated to observe the membrane repairing process. We added the polarization pattern control system to a nonlinear Raman microscopy system to detect membrane proteins by control the direction of electro-magnetic filed on the focus spot. The possibility of the selective detection of ordered molecules was confirmed with the imaging of liquid crystal.