Engineering three dimensional hepatic tissue with microvascular networks in vivo

具有微血管网络的高功能三维（3-D）组织的创造一直热切期待再生医学，然而，这种重建的技术还不够成熟。我们研究的最终目标是创造人类高功能和复杂的组织。为了实现这一最终目标，我们首先在三维纤维连接蛋白-胶原凝胶中植入人脐静脉内皮细胞（HUVECs）和人间充质干细胞（hMSC），并在体外重建了人三维微血管。接下来，我们通过将一小块嵌入HUVEC和hMSC的纤维连接蛋白-胶原凝胶移植到小鼠颅窗中，在体内重建了这种人类微血管，旨在建立具有微血管网络的人类3D肝组织。

Creation of the highly functional three dimensional (3-D) tissue with microvascular networks has been eagerly waited for regenerative medicine, however, the technology of such reconstitution is not established enough. The final goal of our study is creation of human highly functional and complicated tissues. To reach this final goal, we first seeded human umbilical-vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSC) in a three-dimensional fibronectin-collagen gel and reconstituted human 3D microvessels in vitro. Next, we reconstituted this human microvessls in vivo by transplanting a small piece of this fibronectin-collagen gel embedded HUVEC and hMSC in mouse cranial windows and aimed at the creation of human 3D hepatic tissue with microvascular network.