Quantitative Evaluation of myofilaments remodeling during relaxation-contraction cycling in smooth muscle cells.

为了定量评价平滑肌细胞肌丝重塑与力学性能的关系，我们采用电子显微镜、荧光共振能量转移（FRET）分析和常规电泳技术，测量了相同的剥皮（细胞膜渗透）盲带绦虫制剂的收缩力和肌丝结构的变化。此外，对剥皮盲带绦虫的x射线衍射研究显示，在收缩-松弛周期中，晶格结构的动态变化源于肌丝的排列。

To quantitatively evaluate the relation between myofilament remodeling and mechanical properties in vertebrate smooth muscle cells, by using electron microscopy, florescent resonance energy transfer (FRET) analysis and conventional electrophoresis techniques, we measured both the contractile force and changes in the myofilament structure of the same preparations of skinned (cell membrane permeabilized) taenia cecum. Also an X-ray diffraction study using skinned taenia cecum showed dynamic changes in the lattice structure originated from myofilament arrangement during contraction-relaxation cycles.