Development of a highly sensitive protein detection method using substrates with nano-scale strcture.

在玻璃衬底上构建了荧光增强结构。首先，在载玻片上沉积银膜。六甲基二硅氧烷等离子体聚合膜(PPF)随后被沉积成具有不同膜厚的介电膜。对荧光团标记的蛋白质进行斑点，并测量底物的荧光强度。因此，当PPF厚度约为56 nm时，荧光强度可增强约13倍，这表明未来有可能进行高灵敏的免疫分析。

Fluorescence enhancement structure was built up on the glass substrate. First, the Ag film was deposited on the slide glass. The hexamethyldisiloxane plasma-polymerized film (PPF) was subsequently deposited as a dielectric film with various film thickness. The fluorophore-labeled protein was spotted, and the fluorescence intensity from the substrate was measured. As a result, the fluorescence intensity could be enhanced about approximately 13-fold when the thickness of PPF was approximately 56nm, suggesting the future possibility of highly sensitive immunoassay.