Identification of the gene for a sporeless mutation and its application in edible mushroom breeding.

本研究在DNA标记的基础上构建了肺侧耳无孢子突变体的遗传连锁图谱，并确定了无孢子突变体的基因座。此外，我们还获得了与该基因座紧密连锁的DNA标记。这些结果为开展无孢菇的高效繁育奠定了基础。此外，我们还克隆了与该突变相关的区域，并根据连锁图谱的信息确定了该区域的全部核苷酸序列。通过比较突变型和野生型的序列，我们发现了几个基因结构上的差异。今后，我们将通过互补试验等手段，鉴定导致该真菌无孢子化突变的基因。

In this study, we constructed the genetic linkage map of the sporeless mutant of Pleurotus pulmonarius based on the DNA markers, and determined the locus of sporeless mutation. Moreover, we obtained the DNA markers which are very tightly linked to this locus. These results enable to carry out the efficient breeding of a sporeless mushrooms. In addition, we cloned the region related to this mutation, and based on the information of linkage map we determined the whole nucleotide sequences of this region. By the comparison of the sequences from mutant and wild type, we found the differences in several gene structures. In future, we will identify the gene that causes the sporeless mutation of this fungus by using the complementation test and so on.