Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Transdifferentiate Into Neural Cells And Promote Functional Recovery From Spinal Cord Injury-Induced Motor Dysfunction In Rodents

从脂肪组织中分离成熟脂肪细胞并在天花板培养中生长时，可转化为无脂滴的成纤维细胞样细胞。这些成纤维细胞样细胞被称为去分化脂肪(DFAT)细胞，可以增殖，也可以重新分化为成熟的脂肪细胞。DFAT细胞表达Nestin、NSE、βⅢ微管蛋白、神经营养因子、S100、胶质纤维酸性蛋白和CNPase等神经标志物。将DFAT细胞移植到脊髓损伤(SCI)诱导的啮齿动物体内，可显著恢复后肢功能障碍。在注射部位检测到移植细胞，其中一些细胞表达βIII微管蛋白。这些结果表明，DFAT细胞可以转分化为神经细胞，并有助于促进功能恢复。这些发现还表明，成熟脂肪细胞可能成为治疗中枢神经系统疾病的细胞替代疗法的新来源。

When mature adipocytes were isolated from adipose tissue and grown in ceiling culture, transformation into fibroblast-like cells without lipid droplets occurred. These fibroblast-like cells, termed de-differentiated fat (DFAT) cells, could proliferate and could also redifferentiate into mature adipocytes. DFAT cells expressed neural markers such as nestin, NSE, βIII tubulin, NF, S100, GFAP and CNPase. Allografting of DFAT cells into spinal cord injury (SCI)-induced rodents led to significant recovery from hindlimb dysfunction. Grafted cells were detected at the injection site, and some of these cells expressed βIII tubulin. These results indicate that DFAT cells can transdifferentiate into neural cells, and contribute to the promotion of functional recovery. These findings also suggest that mature adipocytes could become a new source for cell replacement therapy to treat central nervous system disorders.