Effect of durotaxis-dependent cell movement in wound on development of keloid

瘢痕疙瘩是一种皮肤纤维化疾病，可以由皮肤上的轻微损伤引起，比如痤疮或耳洞，也可以由烧伤等严重损伤引起。瘢痕疙瘩的特征是细胞外基质如胶原蛋白的过度沉积，其组织学形态具有特征性。瘢痕疙瘩多见于胸部、肩部或肘关节等部位，容易受到机械拉伸的影响。因此我们认为瘢痕疙瘩成纤维细胞对机械应力的反应可能与正常皮肤成纤维细胞不同。我们用细胞拉伸仪（NC-500）分析了成纤维细胞对机械应力的敏感性。NC-500有硅腔，在硅腔上培养成纤维细胞并划伤（我们称之为伤口修复划伤模型）。我们发现伤口的修复有垂直划伤和水平划伤之分。此外，敲除CD151和EWI-F四葡聚糖网痣可诱导细胞脱离培养装置。这种作用在角质形成细胞中比在角质样成纤维细胞和正常成纤维细胞中更高。因此，我们认为四葡聚糖网痣影响皮肤伤口愈合。

Keloids are skin fibrotic conditions that can be caused by minor insults to skin, such as acne or ear piercing, or by severe injuries such as burns. Keloids are characterized by an overabundant deposition of extra-cellular matrix such as collagen, and their histologic morphology has a characteristic manner. Keloid occurs more often on chest, shoulder or elbow-joint etc. which is easily affected by mechanical stretch. So we consider that the response to mechanical stress of fibroblast derived from Keloid may be different from that of normal skin fibroblast. We analyzed the sensitivity to mechanical stress using cell stretch apparatus (NC-500) which has silicon chambers on which the fibroblasts are cultured and scratched (We called wound repair scratch model). We found that wound repair is deffernted vertical and horizontary scratch. Furthermore knockdown of CD151 and EWI-F tetraspanin web moles induced detachment of cells from a culture apparatus.This effect is higher in keratinocytes than in keroid fibroblasts and normal fibroblasts. Therefore, we suggest that tetraspanin web moles affect skin wound healing.