Search for target molecule for immunotherapy based on Porphyromonas gingivalis genome analysis

牙龈卟啉单胞菌（Porphyromonas gingivalis，P.gingivalis）是牙周炎的重要致病菌，具有多种致病因子，根据其菌毛A（fimA）基因型可分为I-V和Ib型。流行病学研究表明，侵袭性牙周炎患者中存在fimA Ⅱ型菌株，且与侵袭性牙周炎的关系最为密切，同时fimA Ⅱ型菌株在心血管疾病患者中的检出率最高。本研究利用基因组学和蛋白质组学技术寻找Ⅱ型牙龈卟啉单胞菌的新病原体，并构建了中和其毒力因子活性的鼠源单克隆抗体（MAb），以开展被动免疫治疗。

Porphyromonas gingivalis (P.gingivalis), an important periodontal pathogen in periodontitis, possesses a variety of virulent factors, and are classified into six genotypes (types I-V and Ib) based on the genotype of fimbriae A (fimA). The epidemiological studies shown that fimA II strains is harbored in most of the aggressive periodontal patients, and are thought to be strongest related to aggressive periodontitis, furthermore, fimA II is most frequently detected in patients with cardiovascular disease. In this report, to develop passive immunotherapy, we tryied to search the new pathogen of this type II P.gingivalis using the genomic and proteomic techniques, and then mouse monoclonal antibody (MAb), which neutralizes virulent factor activity, is constructed.