Structure-based Engineering of Enzymes Involved in the Biosynthesis of Secondary Metabolites

III型聚酮化合物脱氢酶催化结构多样的、具有生物活性的植物多酚的生物合成。根据酶的X射线晶体结构，我们合理地设计并产生了一组具有新的和扩展的催化功能的定点突变酶。此外，通过利用酶的混杂底物特异性，我们成功地生产了一系列化学和结构上不同的非天然新化合物。

Type III polyketide synthases catalyze the biosynthesis of structurally diverse, biologically active plant polyophenols. On the basis of the X-ray crystal structures of the enzymes, we rationally designed and generated a set of site-directed mutant enzymes with novel and expanded catalytic functions. Further, by exploiting the promiscuous substrate specificities of the enzymes, we succeeded in production of an array of chemically and structurally divergent unnatural novel compounds.