Pursuit of a new system for a high throughput screening of drugs to develop a new strategy of prevention and treatment of ATL

基于表达谱分析，我们制备了一个包含12个ATL细胞异常表达水平基因的基因面板。同时用QuantiGene Plex在短时间内检测这些基因的表达水平。根据公式计算，将测量值转化为“ATL评分”。“ATL评分”用于监测药物对ATL细胞及其衍生细胞系的影响，尽管由于成本原因，使用该系统的实验受到限制。在该系统的建立过程中，我们获得了HTLV-1携带者和ATL患者外周血特异性基因表达水平的详细信息，为HTLV-1感染细胞转化过程中表达水平的变化提供了信息。对一些化学物质对ATL细胞系的影响进行了常规分析，为这些分子的ATL特异性活性提供了有趣的信息。

We prepared a gene panel of 12 genes that show abnormal expression levels on ATL cells based on the expression profile analyses. The expression levels of these genes were measured by QuantiGene Plex simultaneously and in a short time. The measured values were transformed into an "ATL score" by calculation base on a formula. "ATL score" was used to monitor effects of drugs against ATL cells and cell lines derived from them, although experiments using this system were limited because of the cost. In the process of establishing this system, we obtained detailed information about the expression levels of specific genes in PBMCs of HTLV-1 carriers and ATL patients, which provided information about the changes in expression levels in the course of transformation of HTLV-1-infected cells. Conventional analyses were done on some chemicals as to the effects on the ATL cell lines, which provided interesting information about the ATL-specific activities of these molecules.