Molecular breeding of transgenic tree for phytoremediation of aromatic compounds

许多白杆真菌产生许多木质素过氧化物酶。其中，糙皮侧耳MnP 2因其强的过氧化物酶活性而被报道降解难降解的多环芳烃化合物。我们将白杨纤维素酶信号肽与mnp2成熟区的融合基因导入拟南芥中，该融合基因受叶特异性或根特异性启动子控制。一些转基因植物表现出降解双酚A的能力。这为利用分子育种技术培育表达mnp2基因的转基因树提供了可能。我们还将CaMV 35 S启动子控制下的土壤细菌Ralstonia eutropha NH 9的氯儿茶酚二氧合酶基因导入杨树（杂种白杨），并在转基因杨树中成功表达。

Many white-rod fungi produce many lignin peroxidases. Among them, Mnp2 of Pleurotus ostreatus was reported to degrade recalcitrant polyaromatic compounds because of its strong peroxidase activity. We introduced a fusion gene encoding poplar cellulase signal sequence and mnp2 mature region under the control of leaf specific or root specific promoter into Arabidopsis thaliana. Several transgenic plants showed the ability to degrade BisphenolA. This suggests the possibility of molecular breeding of transgenic tree which can express the mnp2 gene. We also introduced a chlorocatechol dioxigenase gene of a soil bacterium Ralstonia eutropha NH9 under the control of CaMV35S promoter into poplar plant (hybrid aspen) and this gene was successfully expressed in the transgenic poplar plant.