Electrochemical DNA Devices for Gene Typing and Biomolecular Sensing

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We have prepared several DNA devices that involve anthraquinone or ferrocene as a redox reporter at the specific position of DNA. The devices exhibit excellent performances in the sensing of DNA sequences or biomolecules such as ATP and proteins. Our method for the DNA sensing is based on strand exchange reactions of the redox-probe oligonucleotide with target DNA at the surface of the devices. We have shown that a strand exchange approach is applicable in aptamer based biosensors. During the grant research period, we have also succeeded the synthesis of redox-oligonucleotides via the pai-electron conjugation between the reporter and the nucleoside base. We anticipate that the redox-oligomers would be used for the new systems in detection of DNA mutation and in electron migration on DNA wires.