Molecular mechanism of cyanobacterial circadian clock using the reconstituted in-vitro oscillator.

生物钟可以在ATP存在下通过KaiA、KaiB和KaiC蛋白在体外重建。在这项研究中，LdpA假定是一个氧化还原传感器在蓝藻进行了生化分析。氧化还原状态改变了分子的结构。此外，发现KaiABC重构系统的pH对循环的长度有影响。细胞内pH和LdpA两个因子都参与了光对细胞周期长度的影响。

The circadian clock can be reconstituted in vitro by the KaiA, KaiB, and KaiC proteins in the presence of ATP. In this study, LdpA presumed to be a redox sensor in cyanobacteria was analyzed biochemically. The structure of the molecule was changed by a redox state. Moreover, it was found that pH of the KaiABC reconstitution system have an effect on the length of the cycle. The two factors, pH in the cell and LdpA, could get involved in the change of the period length the cycle by light.