Establishment of the technique for sperm cryopreservation in Okinawan native Agu pig

猪精子冷冻保存技术的建立是对稀有的冲绳本土阿古猪进行有效繁育的必要条件。在本研究中，在冷冻扩展剂中添加抗坏血酸2-Ο-α-葡萄糖苷（AA-2G）和低密度脂蛋白（LDL），分别通过保护精子在冷冻保存过程中免受细胞外氧化应激和冷休克损伤，减少了解冻后阿古精子的质膜损伤。此外，人工抗细胞死亡蛋白（PTD-FNK蛋白）可以防止线粒体功能障碍导致的细胞凋亡。因此，这些结果表明，通过本研究建立的技术，可以有效地改善脆弱的阿古精子的解冻后品质。

The technical establishment of boar sperm cryopreservation isindispensable for effective breeding of the scarce Okinawan native Agu pig. In this study, the addition of ascorbic acid 2-Ο-α-glucoside(AA-2G) and low-density lipoprotein(LDL) tothe freezing extender reduced the plasma membrane damage in post-thaw Agu sperm as aconsequence of the protection of sperm against extracellular oxidative stress and cold shockdamage, respectively, during cryopreservation. Moreover, treatment with an artificial anti-cell death protein(PTD-FNK protein) prevented the mitochondrial dysfunction leading to the apoptotic-cell death during cryopreservation. Therefore, these results indicate that the post-thaw qualities of fragile Agu sperm is effectively improved throughthe established techniques in this study.