Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A.

本研究的目的是观察组蛋白脱乙酰酶抑制剂(HADCI)和肌动蛋白聚合抑制剂LATA(LATA)的激活后处理对转基因小猪体细胞核移植(SCNT)胚胎体外和体内发育的影响。电激活后的SCNT胚胎分别用或不用LATA处理，并进行体外培养。囊胚形成率显著高于对照组(p&lt；0。HADCi和0。5μM LATA明显高于对照组。用HDACi和LATA处理的胚胎移植后，从受体子宫获得胎儿和仔猪。本研究首次表明，HDACi和LATA的后激活处理对提高转基因小型猪胚胎的体外和体内发育能力是有效的。

The objective of this study was to examine the effects of postactivation treatment of histone deacetylase inhibitors(HADCi) and an actin polymerisation inhibitor, latrunculin A(LatA) on in vitro and in vivo development of somatic cell nuclear transfer(SCNT) embryos derived from gene^modified Clawn miniature pig cells. After electric activation, SCNT embryos were treated with or without LatA, and cultured in vitro. The rate of blastocyst formation was significantly higher(p<0. 05) in SCNT embryos treated with HADCi and 0. 5μM LatA than that in control. In addition, fetuses and piglets were obtained from recipient uteri after transfer of embryos treated with HDACi and LatA. The results of this study showed for the first time that postactivation treatment with HDACi and LatA was effective to improve in vitro and in vivo developmental capacity of gene-modified cloned miniature pig embryos.