Construction of artificial poly-clonal gamma globulin to control HCV infection and development of assay system

利用来自外周血的cDNA，构建了由VH、CH1和铰链区组成的178个人工多克隆γ球蛋白。在大肠杆菌中表达的重组蛋白，以毫克为单位进行提取、纯化和回收。SCGK/J小鼠是一种炎症性疾病模型，虽然该菌株不是肝脏疾病模型，但用重组多克隆γ球蛋白进行了治疗性治疗。经一定剂量重组蛋白治疗后，检测血象及部分细胞因子。外周血淋巴细胞及部分炎性细胞因子呈剂量依赖性降低，部分抑制性细胞因子呈剂量依赖性升高。提示本研究构建的重组多克隆γ球蛋白有可能应用于肝脏炎症性疾病的治疗。

178 clones of artificial poly-clonal gamma globulin, which consist of VH, CH1 and hinge region, was constructed from cDNA originated from peripheral blood. The expressed recombinant proteins in E. coli, were extracted, purified and recovered in milligram order. SCGK/J mouse, which is an inflammatory disease model, although the strain is not a model of hepatic disease, were examined for therapeutic treatment with the recombinant poly-clonal gamma globulin. After the therapeutic treatment in some dose of recombinant protein, hemogram and some cytokines were assayed. The level of peripheral blood lymphocyte and some inflammatory cytokines were decreased in dose dependent manner, and some suppressive cytokines were increased. It was suggested that the recombinant poly-clonal gamma globulin, constructed in this study, would be possible to apply to the therapy for hepatic inflammatory disease.