Metabolic and Kinetic Study of D-Aspartate-Containing Protein Caused by Oxidative Stress

氧化应激导致蛋白质中D-天冬氨酸残基(D-Asp)的形成和积累，导致蛋白质变性，从而导致与衰老相关的错误折叠疾病，如阿尔茨海默病。我们试图鉴定一种未知的蛋白酶选择性地降解含有D-天冬氨酸的蛋白，即D-天冬氨酸内肽酶(DAEP)，并最终对其进行纯化。因此，在哺乳动物的长寿过程中，DAEP似乎在生理上充当了对抗有毒D-Asp蛋白的质量控制系统。

The formation and accumulation of D-aspartate residue (D-Asp) in proteins caused by oxidative stress leads to denaturation of proteins, and is consequently responsible for aging-related misfolding diseases such as Alzheimer's disease. We sought to identify that an unknown protease selectively degrades the D-Asp-containing protein, namely D-aspartyl endopeptidase (DAEP), and finally purified it. DAEP therefore seems to physiologically serve as the quality control system against the noxious D-Asp-containing protein in the long life span of mammals.