Contribution of new cancer related gene OCIAD2 to carcinogenesis and malignant alteration of pulmonary adenocarcinoma

制备了抗早期浸润性肺腺癌高表达的OCIAD结构域2（OCIAD 2）重组肽的多克隆抗体，检测了133例小肺腺癌和118例粘液性卵巢癌中OCIAD 2的表达。OCIAD 2表达与肺腺癌预后无相关性。然而，尽管几乎所有正常肺组织对OCIAD 2均为阴性，但许多肿瘤细胞被OCIAD 2抗体染色，因此，OCIAD 2被认为是肺腺癌特异性癌相关抗原。另一方面，OCIAD 2的表达与卵巢粘液性肿瘤的恶性变化显著相关。组织学上，OCIAD 2在肿瘤细胞的乳头状形成或间质浸润中强表达。推测OCIAD 2可能分泌于粘液性卵巢癌患者的腹水或血清中。为了建立OCIAD 2在腹水或血清中的检测系统，我们将OCIAD 2阳性的卵巢肿瘤细胞移植到裸鼠体内，然后制成基因移植瘤小鼠。基因移植肿瘤也显示了OCIAD 2表达。

Polyclonal antibody against the recombinant peptides of OCIAD domain containing 2 (OCIAD2) that was highly expressed in early invasive pulmonary adenocarcinoma was produced and we examined the expression of OCIAD2 in 133 small-sized lung adenocarcinomas and 118 mucinous ovarian carcinomas. There was no correlation between OCIAD2 expression and outcomes for lung adenocarcinoma. However, although almost all normal lung tissue was negative for OCIAD2, a number of tumour cells were stained by OCIAD2 antibody, therefore,OCIAD2 was thought to be lung adenocarcinoma specific cancer related antigen. On the other hand, OCIAD2 expression significantly correlated with malignant alteration in ovarian mucinous tumour. Histologicaly, OCIAD2 was strongly expressed in the papillary formation or the stromal invasion of tumour cells. It was expected that OCIAD2 might be secreted in the ascites or the serum of the patients with mucihous ovarian cancer. In order to construct the detection system for OCIAD2 in the ascites or the serum, we transplanted OCIAD2 positive ovarian tumour cell to the nude-mice, then made the genograft mice. The genograft tumour also revealed the OCIAD2 expression.