Effect of circulating monocyte subsets and endothelial progenitor cells on coronary plaque rupture

单核细胞代表一种异质循环的细胞群体。我们发现在急性心肌梗死患者中，CD14^+CD1^6-CCR2^+和CD14^+CD16^+CX3CR1^+两个不同的单核细胞亚群水平发生了动态变化，这种单核细胞系统的随机图谱可能对心血管疾病(心脏J am Coll)具有临床实用价值。2009年)。稳定型心绞痛(SAP)或急性冠脉综合征患者的早期诊断和风险分层至关重要。我们研究了循环单核细胞亚群水平对冠状动脉易损斑块负荷的影响。首先，我们研究了80例SAP患者单核细胞亚群与冠状动脉斑块易损性的关系，如正重构(重构指数1.05)和/或低CT密度(&lt；35HU)。流式细胞仪检测不同的单核细胞亚群。冠状动脉易损斑块患者CD14+CD16+CX3CR1+单核细胞的相对比例显著高于无斑块患者。单核细胞亚群与重构指数呈正相关，与CT衰减值(动脉粥样硬化)呈负相关。2010)。接下来，我们研究了40例不稳定型心绞痛患者单核细胞亚群计数与冠状动脉纤维帽厚度(FCT)的关系。在基线和9个月后用光学相干断层扫描(OCT)评估非罪魁祸首FCT的变化。FCT变化与CD14^+CD16^+CX3CR1^+单核细胞百分比变化呈显著负相关，而与CD14^+CD16^-CCR2^+单核细胞百分比变化(动脉粥样硬化)无关。2010)。结论：CD14+CD16^+CX3CR1^+单核细胞可作为冠状动脉斑块易损性的新的生物标志物。

Monocytes represent a heterogenous circulating population of cells. We showed that dynamic changes occur in 2 distinct monocyte subset levels (CD14^+CD1^6-CCR2^+ and CD14^+CD16^+CX3CR1^+) in patients with acute myocardial infarction, and that stochastic profiling of this monocyte system may have hold clinical utility with respect to cardiovasculardiseases (J Am Coll Cardiol. 2009). Early diagnosis and risk stratification of patients with stable angina pectoris (SAP), or acute coronary syndrome is of utmost importance. We examined the impact of circulating monocyte subset levels on the burden of coronary vulnerable plaque. First, we investigated the relationship between monocyte subsets and coronary plaque vulnerability, such as positive remodeling (remodeling index >1.05) and/or low CT attenuation (<35 HU), assessed by multi-detector computed tomography (MDCT) in 80 patients with SAP. The distinct monocyte subsets were measured by flow cytometry. The relative proportions of CD14^+CD16^+CX3CR1^+ monocytes were significantly higher in patients with coronary vulnerable plaque than in patients without it. These monocyte subpopulations positively correlated with remodeling index, and negatively with CT attenuation value (Atherosclerosis. 2010). Next, we examined the association of monocyte subset counts with coronary fibrous cap thickness (FCT) in 40 patients with unstable angina pectoris. The changes in the non-culprit FCT were assessed by optical coherence tomography (OCT) at baseline and after 9 months. The percent changes in FCT showed significantly negative correlation with the percent changes in CD14^+CD16^+CX3CR1^+ monocytes, but not CD14^+CD16^-CCR2^+monocytes (Atherosclerosis. 2010). In conclusion, CD14+CD16^+CX3CR1^+ monocytes may serve as a novel biomarker for coronary plaque vulnerability.