Development of a Time-Resolved Infrared Spectrophotometer ～ Elucidation of the proton pumping mechanism ～

我们的红外光谱仪采用飞秒红外激光器作为光源。由于水蒸气使红外光谱具有显着的价值，并且填充了干的N_2气体，因此建造了一个腔体来覆盖光学系统。在时间分辨红外光谱实验中，将SU-8聚合物夹在CaF_2窗口之间，制成了一个尺寸为50×10~(-6)m(光路长度)和400×10~(-6)~(-6)m(流道宽度)的微流动池。利用该系统获得了细胞色素C氧化酶的还原状态和氧化状态的不同光谱。

A femtosecond infrared laser was combined to our infrared spectrometer for light source. A chamber was constructed to cover while optical system because the water vapor makes IR spectra worth remarkably and it was filled with dry N_2 gas. For time-resolved IR experiment, a micro flow cell that dimension has 50 x 10^<-6> m (light path length) and 400 x 10^<-6> m (flow pass width) was made by sandwiched SU-8 polymer between CaF_2 windows. A difference spectra between reduced state and oxidized state of cytochrome c oxidase was acquired by using these system.