Detection of CHOP gene rearrangements in myxoid liposarcoma by PCR and FISH analyses

黏液样脂肪肉瘤具有特异性的融合基因，如TLS/FUS-CHOP。我们尝试使用福尔马林固定石蜡包埋标本，通过RT-PCR检测TLS/FUS-CHOP融合基因转录或FISH检测CHOP基因重排。对比两种分析，FISH比RT-PCR更敏感。同一肿瘤中不同组织学成分（粘液瘤、白色脂肪、棕色脂肪和圆形细胞）的CHOP基因重排相同。提示TLS/CHOP融合基因对黏液样脂肪肉瘤内部的表型表达没有直接影响。

Myxoid liposarcoma has the specific fusion gene such as TLS/FUS-CHOP. We tried to detect TLS/FUS-CHOP fusion gene transcript by RT-PCR or CHOP gene rearrangement by FISH using formalin-fixed paraffin-embedded specimens. Contrasting the two analyses, FISH was more sensitive than RT-PCR. The histologically different components involving myxomatous, white adipose, brown adipose and round cell ones in the same tumor had the identical CHOP gene rearrangement. It suggests that the phenotypic expression of the inside of myxoid liposarcoma are not directly influenced by TLS/CHOP fusion gene.