Establishment of differentiation method of iPS cell derived cardiomyocytes

我们分析了来自小鼠iPS细胞的心肌细胞分化的差异。对纯化的ips源心肌细胞进行RT-PCR分析发现，外源残留转基因表达水平较高的ips源心肌细胞比外源残留转基因表达水平较低的ips源心肌细胞跳动效率较低。外源性转基因的高表达水平可能有助于降低跳动菌落的发生率，阻止向心肌细胞分化。

We analyzed the difference of cardiomyocyte differentiation derived from mouse iPS cells. RT-PCR analysis of the purified iPS-derived cardiomyocytes revealed that iPS-derived cardiomyocytes with higher expression level of residual exogenous transgene had lower beating efficiency compared with that of iPS-derived cariomyocytes with lower expression level of residual exogenous transgene. Higher expression level of exogenous transgene may have contributed to lower incidence of beating colonies, preventing differentiation to cardiomyocytes.