BRM cocktail treatment using PSK and OK432 significantly up-regulates the migration activity in human dendritic cells without losing effective CTL induction

基于树突状细胞（DCs）的癌症免疫疗法广泛应用于多种人类癌症；然而，患者的结果仍然是不可接受的。成功的树突细胞免疫治疗的主要因素之一被认为是维持成熟树突细胞的迁移活性。在本研究中，我们从成熟、迁移和诱导细胞毒性T淋巴细胞的角度评估了蛋白结合多糖PSK对ok432活化的dc的有效性。OK432处理后的树突状细胞具有较高的细胞毒活性，但迁移能力较差。当DCs同时被OK432和PSK处理时，DCs的迁移能力明显高于单独使用OK432，保留了OK432处理的有益效果。结合OK432和PSK的生物反应调节剂鸡尾酒治疗在活化的DC中诱导了高水平的迁移活性，这提示了一种更有效的DC免疫治疗癌症的潜在方案。

Dendritic cells (DCs) based cancer immunotherapies are widely applicable for many kinds of human cancers; however, patient outcomes are still not acceptable. One of the major factors for successful DCs immunotherapy is thought to be the maintenance of the migratory activity of matured DCs. In the present study we evaluated the effectiveness of protein bound polysaccharide PSK on OK432-activated DCs, in terms of maturation, migration and induction of cytotoxic T lymphocytes. OK432 treated DCs induced higher level of cytotoxic activity with poor migration ability. When DCs were treated with both OK432 and PSK, migration ability of the DCs were significantly high as compared to OK432 alone preserving the beneficial effect of OK432 treatment. Biological Response Modifier cocktail treatment with OK432 and PSK induced high level of migration activity in activated DCs, suggesting a potential protocol for more effective DC immunotherapy for cancer.