Induction of pyramidal neuronal cells from bone marrow stromal stemcells and application for autologous transplantation against the cerebral infarction.

我们展示了从大鼠骨髓基质干细胞(BMSC)中诱导出EACC1(谷氨酸转运体)阳性锥体神经元样细胞的方法。转导Notch胞内区(NICD)和垂体腺苷环化酶激活肽(PACAP)基因后的短暂低氧和细胞因子刺激促进了BMSCs向多极神经元样细胞的转分化。将这些细胞移植到梗死脑内一个月后，一些受体细胞存活在宿主脑缺血周围区域，并表达突触相关蛋白。

We demonstrated the way to induce the EACC1(glutamate transpoter)-positive pyramidal neuron-like cells from rat bone marrow stromal stem cells (BMSC). Transient hypoxia and cytokines stimulation following the genes transfection of Notch intracellular domain (NICD) and pituitary adenylate cyclase activating polypeptide (PACAP) promoted the transdifferentiation of BMSCs into the mutipolar neuron-like cells. One month after the transplantation of these cells into the infracted brain, some of recipient cells had survived in the peri-ischemic area of host brain and expressed the synapse-associated proteins.