Generating Synthetic Anti-HPV18 E7 Peptide

构建了研究HPV18E7与Rb相互作用的定量结合分析系统。含有LxCxE基序的多肽结构具有一定的抑制作用，但抑制效果不稳定，通过添加五聚体结构域的调节显示出较强的抑制作用。由于质粒转染率较低，细胞存活率检测未见明显差异。构建了表达五聚体多肽的腺病毒载体，并进行了改进。

Quantitative binding assay system to investigate the interaction between HPV18 E7 and RB was constructed as expression plasmids. Peptide construct including LxCxE motif showed a certain but labile amount of inhibitory effect .Adjustment of the peptide by adding a pentamerization domain showed stronger inhibitory effect. Cell viability assay did not show significant difference due to poor plasmid transfection efficiency. Adenovirus vectors expressing the pentamerized peptides were constructed for improvement.