Promoter hypermethylation and microsatellite instability in patients with head and neck squamous cell carcinoma with other primary sites

在培养的头颈部癌细胞中，28.6%的DAP-K、28.6%的MGMT和14.3%的hMLH 1检测到高甲基化。这些频率与其他研究报告的频率相似。这一发现表明，一些肿瘤相关基因的甲基化在头颈癌的肿瘤发生中起着重要作用。多原发癌患者中高频率MSI患者的比例高于无多原发癌患者（p=0.0310）。因此，MSI似乎是预测头颈癌患者继发性癌症发展的候选生物标志物。

In cultured cells from head and neck cancer, hypermethylation was detected at DAP-K in 28.6%, at MGMT in 28.6%, and at hMLH1 in 14.3%. These frequencies are similar to those reported in other studies. This findings suggest that hypermethylation of several tumor-associated genes plays an important role in tumorgenesis of head and neck cancer. The proportion of high-frequency MSI patients was higher in cases with multiple primary cancers than in those without them (p=0.0310). MSI thus appears to be a candidate biomarker for prediction the development of secondary cancer in head and neck cancer patients.