Preparation of CCP antibodies library in Rheumatoid Arthritis and its clinical application

在类风湿性关节炎（RA）中，已报告与含瓜氨酸的环肽结合的抗体的出现与RA的发作及其疾病进展相关。我们推测，CCP抗体自身抗原的鉴定可能有助于阐明RA中CCP抗体产生的诱导机制及其病理作用，从而可能揭示RA关节破坏的进展机制。为此，我们开发了ISAAC系统，在淋巴细胞芯片上识别产生自身抗体的人淋巴细胞。本研究应用ISAAC系统从自身免疫性疾病患者外周血中获得了自身抗体，并尝试应用ISAAC系统从RA患者中获得CCP抗体。

In rheumatoid arthritis (RA), appearance of antibodies that bind to cyclic peptide containing citrulline has been reported to be correlated with the onset of the RA and its disease progression. We have hypothesized that the identification of auto-antigens of CCP antibodies might clarify the mechanisms of the induction of CCP antibody production and its pathological function in RA, which may lead to reveal the mechanisms of the progression of joint destruction in RA. To this end, we have developed the ISAAC system that identifies auto-antibody-producing human lymphocytes on lymphocyte-chip. By using ISAAC system, we have obtained auto-antibodies from the peripheral blood of patients of autoimmune diseases, and tried to apply ISAAC system to obtain CCP antibodies from RA patients.