Investigation of differentiation of extrapancreatic stem cells to pancreatic β-cells

干细胞分化为胰腺ss细胞，其产生和分泌胰岛素，已被几项实验研究证实，并且正在积极研究将这种分化的细胞移植到糖尿病患者体内。因此，这种干细胞疗法将需要大量分化的细胞以获得足够的疗效。我们证明，球状体的形成和悬浮培养的ss-细胞增强胰岛素分泌能力和敏感性的ss-细胞磺脲类药物，如甲苯磺丁脲通过胰岛素分泌相关分子的功能变化。

The differentiation of stem cells to pancreatic ss-cells, which produce and secrete insulin, is confirmed by several experimental studies, and the transplantation of such differentiated cells into patients with diabetes is actively studied. Such stem cell therapy will therefore require a large number of differentiated cells to obtain sufficient curative effects. We demonstrated that spheroid formation and suspension culture of ss-cells enhanced the insulin secretory capacity and sensitivity of ss-cells to sulfonylurea drugs such as tolbutamide via functional changes in insulin-secretion-related molecules.