Studies on the operative technique and the cytokines required to reproduce a normal bone-ligament junction in the knee ligament reconstruction

在大鼠的免疫组织学研究中，虽然在胎生时期韧带插入部位完全双极化为产生I型胶原的韧带细胞和产生II型胶原的表皮软骨细胞，但在出生后，这两个细胞都有规律地向纤维方向重新排列。在边界部分，似乎一部分韧带细胞转化为软骨细胞，一部分软骨细胞转化为韧带细胞，显示Sox9。(2)虽然肌腱-骨连接处为纤维结合，但当我们将常规骨隧道法的操作方法改为不使用骨隧道的操作方法时，锚定纤维的方向与移植肌腱的机械轴一致。(3)将具有多能性的骨髓间充质干细胞和/或实验-1认为是关键因素之一的sox9引入实验-2建立的重建模型中，发现部分个体通过II型胶原层形成了类似于正常骨-肌腱连接的软骨骨-肌腱连接。

On immunohistological search of the rat, although ligament insertion site is completely bipolarized into the ligament cell which produces type I collagen at a viviparous period and the epiphysial cartilage cell which produces type II collagen, both cells re-arrange regularly to a fibrous direction after birth. In the boundary part, it seemed that a part of ligament cell transmuted into the cartilage cell and a part of cartilage cell transmuted into the ligament cell, with revelation of Sox9. (2) A direction of the anchor fiber came to agree with the mechanical axis of the grafted tendon when we changed the operation method from the conventional bone-tunnel method to our original method without bone-tunnel, although the tendon-bone junction was a fibrous union. (3) The cartilaginous bone-tendon junction through the layer of type II collagen which resembled to a normal bone-tendon junction was found by some individuals, when the bone marrow-derived mesenchymal stem cell which has multipotency and/or Sox 9 which is considered to be one of the key-factors by the experiment-1 were introduced into the our reconstruction model which established by the experiment-2.