Role(s) of pepstatin A-sensitive aspartic proteinases in osteoclast differentiation

我们研究了天冬氨酸蛋白酶家族在小鼠体外破骨细胞分化模型中的作用。组织蛋白酶E基因敲除小鼠骨髓巨噬细胞培养物中的破骨细胞生成受到显著抑制，可能是通过升高RANKL诱导的caspase 3活化。我们还研究了napsins的比较遗传和生化分析。Napsin A在小鼠骨髓细胞中内源性表达，重组蛋白对几种合成的天冬氨酸蛋白酶底物具有胃蛋白酶抑制剂-A敏感活性。

We investigated the roles of aspartic proteinase family in mouse in vitro osteoclast differentiation model. Osteoclastogenesis in bone marrow macrophage culture from cathepsin E-knockout mice were significantly suppressed, probably through elevating the RANKL-induced caspase 3 activation. We also studied the comparative genetic and biochemical analyses of napsins. Napsin A is endogenously expressed in mouse bone marrow cells and recombinant proteins possess pepstatin-A sensitive activities against several synthetic aspartic proteinase substrates.