Novel pulp treatment agent with stimulator for pulp regeneration

本研究的目的是分析促进牙髓血管形成、牙髓伤口愈合和牙髓再生的蛋白质，以开发在牙髓损伤或牙髓炎时更早、更安全地促进牙髓愈合的蛋白质治疗方法。虽然在研究之初计划对大鼠牙髓创面愈合模型进行质谱分析系统地分析牙髓刺激因子，但在大鼠切牙牙髓损伤后即刻、牙髓创面愈合过程中及牙髓愈合后从牙髓组织中获取的蛋白质量不够，敏感性不高。因此，二维电泳分析没有检测到一个特殊的斑点。推测该蛋白促进牙髓创面愈合可能具有较高的迁移作用。采用TAXIScan-FL体外水平趋化实验，考察了多种迁移因子及其浓度梯度对人牙髓干细胞的迁移作用。BDNF对迁移的影响最大，SDF-1和bFGF对迁移的影响也相对较高，GDNF、VEGF、MMP3和G-CSF逐渐促进迁移。最终浓度为10ng/μl时，迁移细胞数量最多。其中，G-CSF作为牙髓刺激因子应用于海绵切除的牙髓，因为它已经被批准为一种药物，其作用是将造血干细胞从骨髓动员到外周血。因此，在100ng中几乎看不到这种效果。它计划改变密度和支架，并在未来进行类似的检查。

The purpose of the present study was to analyze the protein that promotes vascularization, pulp wound healing and pulp regeneration to develop the protein therapy that promotes dental pulp healing earlier and safer at the time of pulp injury or the pulpitis. Though it was scheduled to analyze systematically the pulp stimulating factor by Mass spectrometry analyses in the rat dental pulp wound healing model at the beginning of the research, the amount of the protein obtained from the the dental pulp tissue immediately after rat incisor pulp injury, during pulp wound healing process and after pulp healing was not enough and the sensitivity was not high. Therefore, a special spot was not detected by the two-dimensional electrophoretic analyses. It was speculated that the protein to enhance pulp wound healing may have high migratory effect. The migratory effect of the variety of migration factors and their concentration gradient was examined in the human dental pulp stem cells in the horizontal chemotaxis assay by TAXIScan-FL in vitro. BDNF had the highest effect, SDF-1 and bFGF had also comparatively higher effect on migration, and GDNF, VEGF, MMP3, and G-CSF stimulated migration gradually. The final concentration, 10ng/μl was the highest in the number of migrating cells. Among these, G-CSF was applied on the amputated pulp with spongel to examine the effect as the pulp stimulating factor, because it had already been approved as a medicine by the effect of the mobilization of the hemopoietic stem cells from the bone marrow to the peripheral blood. As a result, the effect was hardly seen in 100ng. It is scheduled to change the density and scaffold and to examine it similarly in the future.