Development of new and safer denture cleanser with Erythritol

本研究的目的是研制一种新型的、安全的义齿清洁剂，它含有糖醇中的一种“赤藓糖醇”，通过控制牙菌斑来预防龋齿、牙周病、口腔炎和吸入性肺炎。体外实验建立了念珠菌生物膜的形成及灭菌效果评价。采用两种方法在包被I型胶原蛋白的塑料盖盖上形成体外白色念珠菌生物膜，并进行比较：1)酵母氮基(YNB) /100 mM葡萄糖培养基中添加2.5 mM n -乙酰氨基葡萄糖（GlcNAc）；2) BHI培养基。本实验采用了前一种方法。采用扫描电镜图像中CFU的定量计数作为灭菌效果的评价。用含BTC溶液的糖醇：赤藓糖醇、木糖醇和山梨醇处理生物膜后，采用微生物测定法评价其杀菌效果。不含BTC的糖醇没有杀菌效果。含赤藓糖醇BTC的杀菌效果随赤藓糖醇浓度的增加而增强。此外，赤藓糖醇的增强水平是三种糖醇中最高的。糖醇，尤其是赤藓糖醇，增强了灭菌剂对体外念珠菌生物膜的杀菌作用。体内实验，采用随机对照试验和PCR法评价含赤藓糖醇溶液和不含赤藓糖醇溶液对牙周袋的杀菌效果。赤藓糖醇溶液杀菌效果稍好。含糖醇，尤其是赤藓糖醇的灭菌液是一种安全有效的义齿清洁剂。

The purpose of the present study was to develop a new and safer denture cleanser with "Erythritol", one of sugar alcohols for preventing dental caries, periodontal disease, stomatitis and aspiration pneumonia through dental and denture plaque control.In vitro experiment, candidal biofilm formation and assessment of sterilized effect were established. An in vitro Candida albicans biofilm was formed on a plastic coverslip coated with type I collagen following two methods and were compared : 1) yeast nitrogen base (YNB) /100 mM glucose medium supplemented with 2.5 mM N-acetylglucosamine (GlcNAc) ; and 2) BHI medium. The former method was employed in this experiment. The quantitative CFU counting in SEM images was employed as the assessment of sterilized effect. The enhancement of the fungicidal effect was evaluated using microbial assay after treating the biofilm with the test solutions (sugar alcohols : erythritol, xylitol, and sorbitol, each containing BTC solution). No fungicidal effect was observed with sugar alcohols without BTC. Thefungicidal effect of erythritol-containing BTC increased with the erythritol concentration. Further, the level of enhancement of erythritol was the highest in three sugar alcohols. Sugar alcohols, especially erythritol, enhanced the fungicidal effect of sterilized agents toward in vitro candidal biofilms. In vivo experiment, the sterilized effect of the solution with and without erythritol on the sterilization of periodontal pocket was evaluated by Randomized Control Trials and PCR test. The sterilized effect of the solution with erythritol was somewhat higher.The sterilized solution with sugar alcohols, especially erythritol would be a safe and effective denture cleanser.