Fabrication of the artificial tissue by using porous sheets and laser tweezers

为了设计与小组织一样大的细胞簇，我研究了取决于细胞簇形成的细胞信号传导和脂滴积累。将细胞接种在图案基底上，使得可以分别形成具有2-8个细胞的细胞簇。观察到小G蛋白在细胞之间的连接部分强烈活化。此外，观察到累积的脂滴的量随着单个细胞簇中连接的细胞数目的增加而增加。

In order to design cell clusters as large as a small tissue, I investigated the cellular signaling and the lipid droplet accumulation depending on the formation of cell clusters. Cells were plated on a pattern substrate so that cell clusters with 2-8 cells could be formed, respectively. It was observed that small G-proteins activated strongly in the joined part between cells. Moreover, it was observed that the amount of accumulated lipid droplets increased with an increase in the number of joined cell in a single cell cluster.