Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants?

在体内筛选化合物的杀真菌活性（即对无菌培养中生长的真菌的活性）比在植物中进行测试要便宜得多，也容易得多。然而，通常观察到，在体内具有活性的化合物随后在植物中并不被证明具有活性。该项目的目的是调查这种差异的生理、生化和遗传学基础，并试图提供规避问题的工具。预计该项目将产生关于真菌所经历的植物环境的新信息。

It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basis of this discrepancy and to attempt to provide tools that circumvent the problem. It is expected the project will generate novel information on the environment in the plant experienced by the fungus.