Mechanosensing in vascular endothelial cells and its response to the physiological stress environment

本研究表明：I)在共聚焦激光扫描显微镜下，分别用DAF2-DA和Rhod2-DA荧光染料监测培养的牛主动脉内皮细胞（BAEC）在流体中产生一氧化氮（NO）和上调细胞内钙离子浓度（[Ca2+] I）；II)二维（2-D）数值模拟LDL在动脉壁上的对流-扩散运输，加上壁面剪切应力梯度（WSSG）依赖于平滑肌细胞（SMCs）的LDL消耗，结果表明，对于门骨孔下方的细胞，SMCs的LDL摄取对WSSG有很强的依赖性。(3)构建模拟真实分岔解剖的两种不同分岔角度的分岔三维模型，模拟恒定氧浓度壁面边界条件下的稳定血流，结果表明颈总内颈外壁氧壁通量分离的分布和大小与分岔角度有明显的关系。

The present study demonstrated that : I) Exposure of cultured bovine aortic endothelial cells(BAEC) to fluid flow resulted in nitric oxide(NO) production and up-regulation of intracellular calcium ion concentration([Ca2+] i), monitored by fluorescent dyes of DAF2-DA and Rhod2-DA, respectively using a confocal laser scanning microscope, II) A two-dimensional(2-D) numerical simulation of a convective-diffusive transport of LDL in the artery wall coupled with the wall shear stress gradient(WSSG) dependent LDL consumption of smooth muscle cells(SMCs) resulted in a strong dependence of LDL uptake of SMCs on WSSG for cells right beneath the fenestral pores, and III) Construction of a three-dimensional models of bifurcations with two different bifurcation angles mimicking the real bifurcation anatomy to perform simulations of steady blood flow under the wall boundary condition of a constant oxygen concentration, resulted in the significant alternation of the distribution and the magnitude of the oxygen wall flux separation at the outer common-internal carotid wall, depending strongly on the bifurcation angle.