Morphofunctional analyses on the Protein 4. 1-family gene deficient mouse

我们把它描述为4。1G与膜相关鸟苷酸激酶（MAGUK）家族成员、膜蛋白棕榈酰化6（MPP6）和免疫球蛋白超家族细胞粘附分子CADM4共定位于SLI。在4。在g基因敲除小鼠中，MPP6和CADM4大多定位在许旺细胞核附近的细胞质中，表明蛋白质转运异常。4岁时发生特殊语言障碍形状改变。与野生型小鼠比较，说明CADM4对粘附的功能意义。另一项免疫沉淀实验显示MPP6与4相互作用。1 g。这些发现表明，4。1G在MPP6和CADM4靶向SLI中具有特殊作用。g - mpp6 - cadm4膜骨架复合体可能维持了SLI的结构，作为抵抗外部机械力的储层。

We describe that the 4. 1G colocalized at SLI with a member of the membrane-associated guanylate kinase(MAGUK) family, membrane protein palmitoylated 6(MPP6), and an immunoglobulin superfamily-cell adhesion molecule, CADM4. In 4. 1G-knockout mice, both MPP6 and CADM4 were mostly localized in cytoplasm near Schwann cell nuclei, indicating abnormal protein transport. SLI shape was altered in aged 4. 1G-knockout mouse nerves compared to that of wild-type mice, indicating functional significance of CADM4 for adhesion. Another immunoprecipitation experiment revealed that MPP6 was interacting with 4. 1G. These findings indicate that the 4. 1G has a specific role in targeting of MPP6 and CADM4 to the SLI, and the novel 4. 1G-MPP6-CADM4 membrane skeletal complex probably maintains the structure of SLI as a reservoir against external mechanical forces.