Histological study and LYVE-1 immunolocalization of mouse mesenteric lymph nodes with "In Vivo Cryotechnique".
Histological study and LYVE-1 immunolocalization of mouse mesenteric lymph nodes with "In Vivo Cryotechnique".
复制标题
DOI：
10.1267/ahc.11002
复制
发表时间：
2011-04-28
影响因子：
2.4
通讯作者：
Ohno S
中科院分区：
文献类型：
--
作者：
Bai Y;Wu B;Terada N;Ohno N;Saitoh S;Saitoh Y;Ohno S
“体内冷冻技术”（IVCT）是一种直接冷冻活体动物器官的有力工具，以保持冷冻组织中的生物成分，反映其天然状态。在这项研究中，活体小鼠肠系膜淋巴结直接冷冻与IVCT，我们做了形态学研究和免疫组化分析的透明质酸受体，LYVE-1。在淋巴结中，观察到广泛开放的淋巴窦，许多淋巴细胞沿着被膜下窦粘附于内内皮细胞。LYVE-1在被膜下窦和髓窦的内皮细胞上有明显的免疫定位。传统的包埋前电子显微镜也显示LYVE-1免疫定位沿着内内皮细胞的细胞膜的顶侧和基底侧。通过对LYVE-1、平滑肌肌动蛋白和IV型胶原的三重免疫染色，LYVE-1仅免疫定位于内内皮细胞中，而不在由胶原基质和平滑肌细胞包围的外内皮细胞中。因此，证明了体内淋巴结的功能形态，并且LYVE-1在被膜下窦的内内皮细胞中的免疫定位表明透明质酸掺入淋巴结实质中。
The “in vivo cryotechnique” (IVCT) is a powerful tool to directly freeze living animal organs in order to maintain biological components in frozen tissues, reflecting their native states. In this study, mesenteric lymph nodes of living mice were directly frozen with IVCT, and we did morphological studies and immunohistochemical analyses on a hyaluronic acid receptor, LYVE-1. In lymph nodes, widely open lymphatic sinuses were observed, and many lymphocytes adhered to inner endothelial cells along subcapsular sinuses. The LYVE-1 was clearly immunolocalized at inner endothelial cells of subcapsular sinuses, as well as those of medullary sinuses. Conventional pre-embedding electron microscopy also showed LYVE-1 immunolocalization along both the apical and basal sides of cell membranes of inner endothelial cells. By triple-immunostaining for LYVE-1, smooth muscle actin, and type IV collagen, the LYVE-1 was immunolocalized only in the inner endothelial cells, but not in outer ones which were surrounded by collagen matrix and smooth muscle cells. Thus, the functional morphology of lymph nodes in vivo was demonstrated and LYVE-1 immunolocalization in inner endothelial cells of subcapsular sinuses suggests hyaluronic acid incorporation into lymph node parenchyma.
登录
查看更多内容
影响因子：
3.7
作者：
Jiang S;Bailey AS;Goldman DC;Swain JR;Wong MH;Streeter PR;Fleming WH
通讯作者：
Fleming WH
DOI：
10.1084/jem.192.10.1425
发表时间：
2000-11-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gretz JE;Norbury CC;Anderson AO;Proudfoot AE;Shaw S
通讯作者：
Shaw S
影响因子：
16.8
作者：
Jackson, DG;Prevo, R;Banerji, S
通讯作者：
Banerji, S
影响因子：
5.4
作者：
Pfeiffer, Friederike;Kumar, Varsha;Engelhardt, Britta
通讯作者：
Engelhardt, Britta
影响因子：
7.8
作者：
Banerji, S;Ni, J;Wang, S X;Clasper, S;Su, J;Tammi, R;Jones, M;Jackson, D G
通讯作者：
Jackson, D G