Expression mechanism and pathological significance of Pentraxin 3 in macrophages and neutrophils.

溃疡性结肠炎患者结肠黏膜组织中的中性粒细胞是正五聚蛋白3（PTX 3）蛋白的主要细胞来源，提示PTX 3蛋白可能参与溃疡性结肠炎患者炎症结肠组织细胞介导的免疫防御。发现PTX 3蛋白与乳铁蛋白^+特异性颗粒一起存在于嗜中性粒细胞中。在IL-8刺激后，PTX 3从PMN释放并定位在由挤出的DNA形成的神经元细胞外陷阱（NET）中。巨噬细胞显示颗粒状阳性的PTX 3。在培养物中证实了来自刺激的中性粒细胞和巨噬细胞的PTX 3的分泌。PTX 3在NET上的结合似乎是针对病原体的防御机制。

Neutrophils in colonic mucosal tissue of patients with ulcerative colitis were main cellular source of Pentraxin 3(PTX3) protein, suggesting that PTX3 protein may contribute to cell-mediated immune defense in inflamed colon tissue of patients with ulcerative colitis. PTX3 protein was found to be present together with lactoferrin^+-specific granules localized in neutrophils. Upon IL-8 stimulation, PTX3 is released from PMNs and localized in Neutrophil Extracellular Traps(NETs) formed by extruded DNA. Macrophages showed granular positivity of PTX3. Secretion of PTX3 from stimulated neutrophils and macrophages was confirmed in culture. Binding of PTX3 on NETs appeared to be a defense mechanism against pathogens.