A role of marcrophage colony-stimulating factor (M-C SF) in macrophage diferentiation
巨噬细胞集落刺激因子(M-C SF)在巨噬细胞分化中的作用
基本信息
- 批准号:08670240
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Since the obteopertrotic (op/op) mice are a'mutation within the coding region of the macrophage colony-stimulating factor (M-CSF) gene, it serves as a model for investigating the differentiation mechanism of macrophage populations. The op/op mice were severely monocytopenic and showed marked reduction of osteoclasts and tissu macrophages. After daily M-CSF injection, the numbers of monocytes , tissue macrophages, and osteoclasts were remarkably increased. These results indicate that M-CSF is a potent inducer of the development and differentation of monocyte/macrophages.We have developed a macrophage depletion method using liposome-entrapped clodronate. By this method Kupffer cells were selectively elimnated in mice. Repopulating small macrophages actively proliferrated and the number of Kupffer cells returned to the normal level by day 14. The numbers of macrophage precursors in the liver increased after Kupffer cell depletion. The precursors proliferated and differentiated into Kupffer cells. M-CSF mRNA expression was enhanced in the liver after Kupffer cell depletion. We then examined the expression of M-CSF and its seceptor in hepatic zymosan-induced granulomas of Kupffer cell-depleted mice.In Kpffer cell-depleted mice, granuloma formation was suppressed. Kupffer cell and monocyte-derived macrophage expressed M-CSF and its receptor in control mice. however, the expression of M-CSF and other cytokines was suppressed in Kupffer cell-depleted mice. These finding imply that local production and inflammation. Local production of M-CSF and the presence of macrophege precursors in the milky spots were also confirmed after depleting omental macrophages by intraperitoneal adminstration of this drug. In conclusion, local production of M-CSF provides microenvironments for the differentiation and proliferation of macrophages.
由于OP/OP小鼠是巨噬细胞集落刺激因子(M-CSF)基因编码区的突变,因此可作为研究巨噬细胞群体分化机制的模型。OP/OP小鼠表现为严重的单核细胞减少,破骨细胞和组织巨噬细胞明显减少。每天注射M-CSF后,单核细胞、组织巨噬细胞和破骨细胞数量显著增加。这些结果表明,M-CSF是一种有效的单核/巨噬细胞发育和分化的诱导剂。我们发展了一种利用脂质体包裹氯屈膦的巨噬细胞耗竭方法。用这种方法选择性地消除了小鼠体内的Kupffer细胞。小巨噬细胞再生活跃,Kupffer细胞数量在第14天恢复到正常水平。Kupffer细胞耗竭后,肝脏中巨噬细胞前体细胞数量增加。前体细胞增殖分化为Kupffer细胞。枯否细胞耗竭后,肝脏M-CSF mRNA表达增强。然后,我们检测了酵母多糖诱导的Kupffer细胞耗竭小鼠肝肉芽肿中M-CSF及其受体的表达。对照组小鼠Kupffer细胞和单核细胞来源的巨噬细胞表达M-CSF及其受体。然而,在Kupffer细胞耗尽的小鼠中,M-CSF和其他细胞因子的表达受到抑制。这些发现表明,当地的生产和炎症。腹腔给药耗尽大网膜巨噬细胞后,乳斑局部产生M-CSF和巨噬细胞前体的存在也得到证实。综上所述,局部产生M-CSF为巨噬细胞的分化和增殖提供了微环境。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Moriyama H et al.: "Expression of macrophage colony stimulating factor and its receptor in hepatic granulomas of Kupffer cell-depleted mice." Am J Pathol. 150. 2047-2060 (1997)
Moriyama H 等人:“巨噬细胞集落刺激因子及其受体在枯否细胞耗竭小鼠肝肉芽肿中的表达。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Naito M,Nagai H,et al.: "Liposome-encapsulated dichloromethylene diphosphonate induces macrophage apoptosis in vivo and in vitro." J Leukoc Biol. 60(3). 337-344 (1996)
Naito M、Nagai H 等人:“脂质体封装的二氯亚甲基二膦酸盐在体内和体外诱导巨噬细胞凋亡。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Naito M et al: "Ostepetrotic mice (op/op) as an animal model for investigating the biology of colony stimulating factor-1 (CSF-1/M-CSF)." Acta Med Biol. 44. 1-11 (1996)
Naito M 等人:“Ostepetrotic 小鼠 (op/op) 作为研究集落刺激因子 1 (CSF-1/M-CSF) 生物学的动物模型。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Umeda S et al.: "Effects to macrophage colony-stimulating factor(M-CSF)on macrophages and related cell populations in osteopetrosis(op)mouse defective in production of functonal M-CSF protein." Am J Pathol. 149(2). 559-574 (1996)
Umeda S 等人:“巨噬细胞集落刺激因子 (M-CSF) 对骨石症 (op) 小鼠巨噬细胞和相关细胞群的影响,这些小鼠无法产生功能性 M-CSF 蛋白。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yamamoto T et al.: "Repopulation of murine Kupffer cells after intravenous administration of liposome-encapsulated dichloromethylene diphosphonate." Am J Pathol. 149. 1271-1286 (1996)
Yamamoto T 等人:“静脉注射脂质体封装的二氯亚甲基二磷酸酯后小鼠库普弗细胞的重新增殖。”
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- 影响因子:0
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NAITO Makoto其他文献
NAITO Makoto的其他文献
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{{ truncateString('NAITO Makoto', 18)}}的其他基金
Expression mechanism and pathological significance of Pentraxin 3 in macrophages and neutrophils.
Pentraxin 3在巨噬细胞和中性粒细胞中的表达机制及病理意义。
- 批准号:
21590397 - 财政年份:2009
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Pathological study on respiratory infectious diseases in Myanmar
缅甸呼吸道传染病病理学研究
- 批准号:
15406012 - 财政年份:2003
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of phagosome-associated protein (TACO) involved in the intracellular infection of mycobacterium
参与分枝杆菌胞内感染的吞噬体相关蛋白(TACO)分析
- 批准号:
13670207 - 财政年份:2001
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Role of scavenger receptor in the processing of bacterial antigens
清道夫受体在细菌抗原加工中的作用
- 批准号:
11670208 - 财政年份:1999
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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