A study of the effect of a recombinant immunosuppressive factor from parasites and immune-inhibitory mechanisms

利用小麦胚细胞无细胞表达系统，克隆了杏核纤毛虫的免疫抑制因子(ES90)，并合成了重组ES90。然而，重组ES90不能抑制巨噬细胞一氧化氮的产生。旋毛虫感染小鼠的巨噬细胞表达YM1、FIZZ1和精氨酸酶1的信使RNA。为此，从旋毛虫中克隆了两种类型的过氧化还蛋白(PRX)，PRX-1mRNA的表达具有阶段特异性

An immunosuppressive factor(ES90) from Spirometra erinaceieuropaei plerocercoids was cloned and the recombinant ES90 was synthesized using wheat germ cell free expression system. However, the recombinant ES90 did not inhibit nitric oxide production in macrophages. Messenger RNA of Ym1, FIZZ1, and arginase 1 were expressed in peritoneal macrophages from Trichinella spiralis-infected mice. In connection with this, 2 types of peroxiredoxin(Prx) from T. spiralis were cloned, Prx-1 mRNA expression was stage-specific