possible inhibitory action of omega-3 unsatulated fatty acid on development of childhood asthma

儿童哮喘是一种慢性气道炎症，其特征是炎症细胞的浸润，包括淋巴细胞、肥大细胞、嗜酸性粒细胞和pmn。我们研究了多种生物活性物质包括不饱和脂肪酸代谢物和细胞因子在人和鼠培养的气道上皮细胞中的作用。(1)白三烯（Leukotrienes, LTB4）是一种不饱和脂肪酸物质，可诱导体外培养的人气道上皮细胞表达ICAM-1，提示其在病毒诱导气道炎症过程中发挥重要作用。(2) Poly IC诱导人气道上皮细胞的sema3A。Sema3A增加人PMNs的吞噬功能。(3)气液界面法证实IL-13对培养的小鼠气管上皮细胞有促进增殖的作用。这种增殖作用与表原的新诱导有关，表原是表皮生长因子受体（EGFR）配体家族的一种物质。

Childhood asthma has been known to be a chronic airway inflammation which is characterized by infiltration of inflammatory cells including lymphocytes, mast cells, eosinophils and PMNs. We investigated action of various bioactive substances including unsaturated fatty acid-metabolites and cytokines in human and murine cultured airway epithelial cells.(1) Leukotrienes(LTB4), a substance of unsaturated fatty acid induced ICAM-1 expression on cultured human airway epithelial cells, indicating important roles in the process of virus-induced inflammation of airway.(2) Poly IC induced sema3A in cultured human airway epithelial cells. Sema3A increased phagocytotic function of human PMNs.(3) IL-13 was confirmed to stimulate proliferation of cultured murine tracheal epithelial cells by using air-liquid interface assay. This proliferative action is associated with new induction of epigen, which is a substance of epidermal growth factor receptor(EGFR) ligand family.