Functional analyses of mammalian round spermatid for fertilization

受精的目标是将性基因组融合到卵子的细胞质中。我们用人类和一只实验动物(小鼠、兔子)检测了哺乳动物圆形精子细胞的受精建立能力，这种细胞被认为是极低的。精子功能完成受精后的重大事件精子进入后，卵母细胞激活能力和精子中心体功能均下降。此外，作为补充卵母细胞激活能力的方法之一，我们还建立了小鼠圆形精子细胞与支持细胞的共培养体系。

The goal of the fertilization assumes he fusion of the sex genome in the egg cytoplasm. We examined fertilization establishment ability of the round spermatid of the mammal that was assumed to be extremely low, using human and a laboratory animal (a mouse, rabbit). Sperm function to accomplish a major event of the fertilization after the sperm entry, oocyte activation ability and sperm centrosomal function were found to be decreased. Furthermore, as one of the methods to supplement ability for oocyte activation that decreased with round spermatid, we developed the co-culture system with hormone addition and Sertoli cells of the mouse round spermatid.