Profiling and functional analyses of microRNAs in ovarian theca cells

我们对从新生小鼠卵巢分离的小鼠鞘干细胞和体外分化的鞘细胞进行了miRNA分析。我们通过基于实时pcr的miRNA阵列分析研究了鞘干细胞和分化细胞之间的差异miRNA特征。在卵泡膜细胞分化过程中，30个mirna表达上调，9个mirna表达下调。匠心途径分析表明，这些mirna参与生殖系统疾病、遗传障碍、细胞发育、细胞生长和增殖以及细胞周期。

We performed miRNA profiling of isolated mouse thecal stem cells from neonatal mouse ovary and in vitro differentiating theca cells. We investigated differential miRNA signatures between thecal stem cells and differentiating cells by real-time PCR-based miRNA array analysis. During theca cell differentiation, 30 miRNAs were upregulated, and 9 miRNAs were downregulated. Ingenuity pathway analysis indicates that these miRNAs participate in reproductive system disease, genetic disorder, cellular development, cellular growth and proliferation, and cell cycles.