The search of graded epigenetic change of oral cancer and Neighboring epithelial dysplasia

我们获得了正常组织(N)、上皮异型增生(D)和癌组织(C)的组织标本，并从两例下颌牙龈癌标本中提取了基因组DNA。我们使用Illumina Human Metylation27对DNA甲基化进行了分析，并对结果进行了统计分析。在N组、→D组、→C组中，甲基化水平逐渐升高的基因依次为EPDR1FoxL1、Flj2159、HOXB4、prc、DAPK1、FlJ46156、WDR8、BARHL2、NFAM1。相反，甲基化水平逐渐降低的基因是MEGEA3、GJB7、MFAP2、GPLD1、LOC348645、MEGEA6、FCN2、CCL1、SLC24A2、C9orf84。对于取消个人差异，未来将有必要增加案件。

We obtained a tissue specimen of normal tissue(N), epithelial dysplasia(D) and cancer(C), and extracted genomic DNA from the sample of two carcinoma of mandibular gingiva. We analyzed DNA methylation using illumina HumanMethylation27 and conducted the statistical analysis of the results. The gene which a methylation level rose to progressively in N group→D group→C group was EPDR1, FOXL1, FLJ21159, HOXB4, PRAC, DAPK1, FLJ46156, WDR8, BARHL2, NFAM1. Conversely, the gene which a methylation level decreased progressively was MEGEA3, GJB7, MFAP2, GPLD1, LOC348645, MEGEA6, FCN2, CCL1, SLC24A2, C9orf84. For the cancellation of the individual difference, it will be necessary to increase cases in future.