Development of tooth regenerative therapy applied molecular mechanisms of epithelial-mesenchymal interactions in tooth morphogenesis

在这项研究中，我们试图发展牙齿再生疗法，应用于牙齿形态发生中上皮-间充质相互作用的分子机制。我们用人骨髓或人脂肪组织来源的间充质干细胞作为牙齿再生治疗的细胞来源，并使用低结晶碳酸盐磷灰石作为牙齿再生治疗的支架。结果表明：1)间充质干细胞具有向成牙本质细胞分化的潜能，2)碳酸盐磷灰石在体内被破骨细胞吸收，3)在碳酸盐磷灰石上培养的间充质干细胞向成骨细胞分化。

In this study, we try to develop the tooth regenerative therapy applied to molecular mechanisms of epithelial-mesenchymal interactions in tooth morphogenesis. We used mesenchymal stem cells derived from human bone marrow or human adipose tissue for cell source of tooth regenerative therapy, and used low crystalline carbonate apatite for scaffold of tooth regenerative therapy. As a result, it was revealed that 1) mesenchymal stem cells have potency of odontoblastic differentiation, 2) carbonate apatite was resorbed by osteoclast in vivo, 3) mesenchymal stem cells cultured on carbonate apatite differentiated into osteoblastic cells.